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st6gal1 gfp (Addgene inc)

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Addgene inc st6gal1 gfp
St6gal1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/st6gal1+gfp/pSV2-c-erbB2+(Plasmid+%2316250)/pmc12237403-74-4-10
Average 93 stars, based on 3 article reviews

st6gal1 gfp - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi
Article Snippet: Recombinant DNA reagent , GFP-Golgin84 , PMID: 12538640 , , A gift from M Lowe lab (University of Manchester). .. Recombinant DNA reagent , ST6GAL1-GFP , PMID: 34533190 , RRID: Addgene_162500 , . .. Recombinant DNA reagent , CD8a-CI-M6PR , PMID: 24285343 , , .

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( A and B ) Hierarchical clustering analysis of gene expression patterns of PANC-1 cells grown under fructose substitution conditions for 28 days and the ABCG2-positive subpopulation were performed using R-project. Heat maps of whole gene (A) and carbohydrate transporter, hexokinase, and sialyltransferase gene sets (B) are shown as the log2 value of fold change compared with parental PANC-1 cells. ( C ) Relative mRNA expression of abcg2 , slc2a5 (GLUT5), and st6gal1 were compared with those of unsorted PANC-1 cells. ( D ) Protein levels of ABCG2, GLUT5, and ST6Gal1 in PANC-1 cells cultured in glucose- (G) or fructose-substituted (F) medium; β-ACTIN was used as an internal control. ( E ) Expression levels of abcg2 , slc2a5 , and st6gal1 in the indicated cells. Values are shown as the mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Oncotarget

Article Title: Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructose-responsive manner promotes pancreatic cancer metastasis

doi: 10.18632/oncotarget.13845

Figure Lengend Snippet: ( A and B ) Hierarchical clustering analysis of gene expression patterns of PANC-1 cells grown under fructose substitution conditions for 28 days and the ABCG2-positive subpopulation were performed using R-project. Heat maps of whole gene (A) and carbohydrate transporter, hexokinase, and sialyltransferase gene sets (B) are shown as the log2 value of fold change compared with parental PANC-1 cells. ( C ) Relative mRNA expression of abcg2 , slc2a5 (GLUT5), and st6gal1 were compared with those of unsorted PANC-1 cells. ( D ) Protein levels of ABCG2, GLUT5, and ST6Gal1 in PANC-1 cells cultured in glucose- (G) or fructose-substituted (F) medium; β-ACTIN was used as an internal control. ( E ) Expression levels of abcg2 , slc2a5 , and st6gal1 in the indicated cells. Values are shown as the mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The pCMV6-AC-GFP-ST6Gal1 vector (Origene) was transfected into the indicated cancer cells using polyJet transfection reagents (SignaGen) and incubated in G418 (MDBio)-containing medium.

Techniques: Expressing, Cell Culture

( A ) Sialylated glycan profiles of parental (green) or PANC-1 cells cultured with fructose for 28 days (red) were detected by click-iT metabolic reagent labeling assays. Parental (blue) or PANC-1 cells cultured with fructose for 28 days (purple) were incubated with 400 nM ManNAc for 24 hours and then labeled with Alexa Fluor488 conjugated alkyne as a negative control. ( B ) Representative immunostaining results for ST6Gal1 (green), α2,6-sialylation (red), or α2,3-sialylation (red), as detected by using anti-ST6Gal1 antibody, biotinylated SNA, or MALII lectin, respectively. ( C and D ) Representative flow cytometry analysis showing staining against SNA and MALII in parental and PANC-1 (C) or PK1 cells (D) incubated for 28 days with fructose. Blue lines indicate cells stained with streptavidin-conjugated secondary antibody as negative control. ( E – G ) ST6Gal1 immunoreactivity and hematoxylin counterstaining on pancreas sections from control Elas-CreER;Kras +/LSLG12D mice (E), and mice treated with tamoxifen (F) or tamoxifen/cerulein (G). Mice were fed a normal diet (upper panel) or fructose diet (lower panel).

Journal: Oncotarget

Article Title: Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructose-responsive manner promotes pancreatic cancer metastasis

doi: 10.18632/oncotarget.13845

Figure Lengend Snippet: ( A ) Sialylated glycan profiles of parental (green) or PANC-1 cells cultured with fructose for 28 days (red) were detected by click-iT metabolic reagent labeling assays. Parental (blue) or PANC-1 cells cultured with fructose for 28 days (purple) were incubated with 400 nM ManNAc for 24 hours and then labeled with Alexa Fluor488 conjugated alkyne as a negative control. ( B ) Representative immunostaining results for ST6Gal1 (green), α2,6-sialylation (red), or α2,3-sialylation (red), as detected by using anti-ST6Gal1 antibody, biotinylated SNA, or MALII lectin, respectively. ( C and D ) Representative flow cytometry analysis showing staining against SNA and MALII in parental and PANC-1 (C) or PK1 cells (D) incubated for 28 days with fructose. Blue lines indicate cells stained with streptavidin-conjugated secondary antibody as negative control. ( E – G ) ST6Gal1 immunoreactivity and hematoxylin counterstaining on pancreas sections from control Elas-CreER;Kras +/LSLG12D mice (E), and mice treated with tamoxifen (F) or tamoxifen/cerulein (G). Mice were fed a normal diet (upper panel) or fructose diet (lower panel).

Techniques: Cell Culture, Labeling, Incubation, Negative Control, Immunostaining, Flow Cytometry, Staining

( A ) HPAEC spectra data show monosaccharide composition analysis of monosaccharide content release from cell extract of ABCG2+CD44+ subpopulation of PANC-1 cells cultured in the indicated conditions. ABCG2+CD44+ CTL indicates freshly isolated ABCG2+CD44+ subpopulation without any treatments. ( B ) Representative bar graphs of HPAEC show the peak area results of glucose and ManNAc from the indicated subpopulation incubated in glucose medium for 30 min (upper panel), or fructose and ManNAc peak area from the indicated subpopulation incubated in fructose medium for 60 min (lower panel). Data are from three independent experiments. ( C ) Representative flow cytometry analysis showing staining against SNA and MALII from PANC-1 cells grown in the indicated medium for 14 days. Blue lines indicate cells stained with streptavidin-conjugated secondary isotype control antibody as negative control. ( D ) Expression levels of abcg2 and st6gal1 in the indicated cells. Values are shown as the mean ± SEM, ns indicate non-significant, * P < 0.05, *** P < 0.001.

Journal: Oncotarget

Article Title: Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructose-responsive manner promotes pancreatic cancer metastasis

doi: 10.18632/oncotarget.13845

Figure Lengend Snippet: ( A ) HPAEC spectra data show monosaccharide composition analysis of monosaccharide content release from cell extract of ABCG2+CD44+ subpopulation of PANC-1 cells cultured in the indicated conditions. ABCG2+CD44+ CTL indicates freshly isolated ABCG2+CD44+ subpopulation without any treatments. ( B ) Representative bar graphs of HPAEC show the peak area results of glucose and ManNAc from the indicated subpopulation incubated in glucose medium for 30 min (upper panel), or fructose and ManNAc peak area from the indicated subpopulation incubated in fructose medium for 60 min (lower panel). Data are from three independent experiments. ( C ) Representative flow cytometry analysis showing staining against SNA and MALII from PANC-1 cells grown in the indicated medium for 14 days. Blue lines indicate cells stained with streptavidin-conjugated secondary isotype control antibody as negative control. ( D ) Expression levels of abcg2 and st6gal1 in the indicated cells. Values are shown as the mean ± SEM, ns indicate non-significant, * P < 0.05, *** P < 0.001.

Techniques: Cell Culture, Isolation, Incubation, Flow Cytometry, Staining, Negative Control, Expressing

( A – D ) ST6Gal1 was transiently overexpressed in low invasive pancreatic cancer cells, Pa8 (A–B) and HPAC (C–D). The expression level of st6gal1 was confirmed by real-time quantitative RT-PCR (A and C) and compared with that of cells expressing the control construct (CMV-GFP). The invasive ability conferred by both constructs was verified by matrigel-coated transwell invasion assay (B and D). ( E ) Real-time quantitative RT-PCR was performed to determine the relative mRNA expression of st6gal1 in each clone stably overexpressing ST6Gal1 (clone 23 and 13) as compared with that in clones expressing the control construct (CMV-GFP CTL). ( F ) Protein levels of ABCG2, GLUT5, and ST6Gal1 (right panel) in each clone stably overexpressing ST6Gal1 (clone 23 and 13). ( G ) The invasive abilities of the control and stable clones overexpressing ST6Gal1 are shown as a bar graph. ( H – J ) H&E stain of orthotopic tumor (H), lung (I), and liver (J) sections from mice injected with the ABCG2-positive subpopulation of PANC-1 cells transformed with control vector (CMV-GFP ABCG2+) or ST6Gal1 construct (CMV-ST6Gal1 ABCG2+). Table summarizing the tumorigenicity in the two groups (H). White arrows indicate nodules in the lung (I) or liver (J). Lung lesions are shown as the number of lesions per lobe (I). Liver metastatic incidence is shown (J). Values are shown as the mean ± SEM, ns indicate non-significant, * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Oncotarget

Article Title: Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructose-responsive manner promotes pancreatic cancer metastasis

doi: 10.18632/oncotarget.13845

Figure Lengend Snippet: ( A – D ) ST6Gal1 was transiently overexpressed in low invasive pancreatic cancer cells, Pa8 (A–B) and HPAC (C–D). The expression level of st6gal1 was confirmed by real-time quantitative RT-PCR (A and C) and compared with that of cells expressing the control construct (CMV-GFP). The invasive ability conferred by both constructs was verified by matrigel-coated transwell invasion assay (B and D). ( E ) Real-time quantitative RT-PCR was performed to determine the relative mRNA expression of st6gal1 in each clone stably overexpressing ST6Gal1 (clone 23 and 13) as compared with that in clones expressing the control construct (CMV-GFP CTL). ( F ) Protein levels of ABCG2, GLUT5, and ST6Gal1 (right panel) in each clone stably overexpressing ST6Gal1 (clone 23 and 13). ( G ) The invasive abilities of the control and stable clones overexpressing ST6Gal1 are shown as a bar graph. ( H – J ) H&E stain of orthotopic tumor (H), lung (I), and liver (J) sections from mice injected with the ABCG2-positive subpopulation of PANC-1 cells transformed with control vector (CMV-GFP ABCG2+) or ST6Gal1 construct (CMV-ST6Gal1 ABCG2+). Table summarizing the tumorigenicity in the two groups (H). White arrows indicate nodules in the lung (I) or liver (J). Lung lesions are shown as the number of lesions per lobe (I). Liver metastatic incidence is shown (J). Values are shown as the mean ± SEM, ns indicate non-significant, * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Expressing, Quantitative RT-PCR, Construct, Transwell Invasion Assay, Stable Transfection, Clone Assay, Staining, Injection, Transformation Assay, Plasmid Preparation

( A ) Expression of st6gal1 in the indicated cells. ( B ) Protein levels of ABCG2, GLUT5, and ST6Gal1 from indicated PANC-1 cell lysate were analyzed by western blotting; β-ACTIN was used as an internal control. ( C ) Flow cytometry analysis and SNA staining was performed to show α2,6-sialylation alteration under each condition. ( D ) Summary of the invasive ability of cells expressing each construct. Green lines: comparison with parental control (glucose shCTL); red lines: comparison with fructose control (fructose shCTL); black lines: comparison with parental control. ( E ) Cell viability of the indicated cells was measured by MTT assay with different concentrations of gemcitabine or cisplatin for 72 hours. ( F – H ) Histological analysis of orthotopic tumor (F), lung (G), and liver (H) tissues from mice injected with the indicated cells. Tables summarizing tumorigenicity (F) and liver metastatic incidence (H) of the indicated cells. Quantification of lung lesions detected by histological analysis (G). White arrows indicate metastatic lesions in lung (G) or liver (H) tissues. Values are shown as the mean ± SEM, ns indicate non-significant, * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Oncotarget

Article Title: Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructose-responsive manner promotes pancreatic cancer metastasis

doi: 10.18632/oncotarget.13845

Figure Lengend Snippet: ( A ) Expression of st6gal1 in the indicated cells. ( B ) Protein levels of ABCG2, GLUT5, and ST6Gal1 from indicated PANC-1 cell lysate were analyzed by western blotting; β-ACTIN was used as an internal control. ( C ) Flow cytometry analysis and SNA staining was performed to show α2,6-sialylation alteration under each condition. ( D ) Summary of the invasive ability of cells expressing each construct. Green lines: comparison with parental control (glucose shCTL); red lines: comparison with fructose control (fructose shCTL); black lines: comparison with parental control. ( E ) Cell viability of the indicated cells was measured by MTT assay with different concentrations of gemcitabine or cisplatin for 72 hours. ( F – H ) Histological analysis of orthotopic tumor (F), lung (G), and liver (H) tissues from mice injected with the indicated cells. Tables summarizing tumorigenicity (F) and liver metastatic incidence (H) of the indicated cells. Quantification of lung lesions detected by histological analysis (G). White arrows indicate metastatic lesions in lung (G) or liver (H) tissues. Values are shown as the mean ± SEM, ns indicate non-significant, * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Expressing, Western Blot, Flow Cytometry, Staining, Construct, MTT Assay, Injection

( A ) Serial sections were assessed by immunohistochemical staining with antibodies against ST6Gal1, GLUT5, and ABCG2. Representative images showed the histological features of samples with low or high ST6Gal1 expression. The expression level of ST6Gal1 was determined by scoring, and samples were classified into low and high expression groups. ( B – D ) The scores of ST6Gal1 staining were based on the percentage and intensity of positive immunohistochemical staining on epithelial-like tissue. Representative graphs showing the expression levels of ST6Gal1 in adjacent tissues and in tumor (B); correlations between ST6Gal1 and GLUT5 (C), or ST6Gal1 and ABCG2 (D). Horizontal bars represent the average values. (E-G) Kaplan–Meier survival analysis of the overall survival of patients with pancreatic cancer, stratified by the expression level of the following markers: ST6Gal1 ( E ), GLUT5 combined with ST6Gal1 ( F ), or ABCG2 combined with ST6Gal1 ( G ). * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Oncotarget

Article Title: Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructose-responsive manner promotes pancreatic cancer metastasis

doi: 10.18632/oncotarget.13845

Figure Lengend Snippet: ( A ) Serial sections were assessed by immunohistochemical staining with antibodies against ST6Gal1, GLUT5, and ABCG2. Representative images showed the histological features of samples with low or high ST6Gal1 expression. The expression level of ST6Gal1 was determined by scoring, and samples were classified into low and high expression groups. ( B – D ) The scores of ST6Gal1 staining were based on the percentage and intensity of positive immunohistochemical staining on epithelial-like tissue. Representative graphs showing the expression levels of ST6Gal1 in adjacent tissues and in tumor (B); correlations between ST6Gal1 and GLUT5 (C), or ST6Gal1 and ABCG2 (D). Horizontal bars represent the average values. (E-G) Kaplan–Meier survival analysis of the overall survival of patients with pancreatic cancer, stratified by the expression level of the following markers: ST6Gal1 ( E ), GLUT5 combined with ST6Gal1 ( F ), or ABCG2 combined with ST6Gal1 ( G ). * P < 0.05, ** P < 0.01, *** P < 0.001.

Techniques: Immunohistochemical staining, Staining, Expressing

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